We upgraded the software for the Phenocycler-Fusion I system to the 2.0 version. It implements and enhances tissue detection.
We submitted a shared instrumentation grant to the NIH to purchase an Alpenglow light-sheet microscope.
The ICBM acquired a second Phenocycler-Fusion multiplex imaging system with funding from the CCEH. The system is identical to the existing Phenocycler system acquired in October 2022.
The Leica Stellaris STED grant wasn't funded.
The ICBM has upgraded the Phenocycler (formerly CODEX) to the Phenocycler-Fusion. The Akoya Phenocycler-Fusion multiplex fluorescence imaging system is an integrated robotic fluidics and fluorescence imaging system that supports multiplexed fluorescence imaging of 40+ probes in the same sample, using a proprietary process of repeated sequential imaging and stripping of fluorescently-labeled DNA-barcode probes. This upgrade separates CODEX runs from the Keyence microscope, giving users increased access to that microscope as well. The Fusion microscope is also capable of high throughput slide scanning with filters for Brightfield and the proprietary Opal dyes between 440 nm and 780 nm.
The ICBM acquired a second high-content, high-throughput imaging system. The Molecular Devices IXM XLS microscope is a fully automated fluorescence microscope equipped with a fluidics handling system and a stage incubator for complex dynamic studies of living cells grown in a variety of different formats.
The high-multiplexing CODEX fluorescence system (for imaging up to 40 probes in a tissue at a time) is now in smooth operation. There are now 16 laboratories on campus at various stages of implementing CODEX for their research, seven have invested in developing antibody panels, and four have done 14 runs. Purchase of this system was made possible by generous contributions from the IU CTSI.
The High-End Instrumentation grant that we submitted to the NIH last spring for funds to purchase a Leica Stellaris STED super-resolution microscope scored reasonably well, well enough that we’re anticipating funding next summer. Fingers crossed.